The Jiali Fault on the southeastern margin of the Tibetan Plateau is a key boundary structure for the southeastward extrusion of plateau material. Its geometric distribution and activity are crucial for understanding the tectonic evolution of the plateau and assessing regional engineering risks. However, the precise spatial location and Holocene activity of its southeastern segment (Guxiang to Gongrigabu section) have long been controversial due to rugged terrain and thick vegetation cover. Targeting this key contentious segment, this study integrated multiple methods, including high-resolution remote sensing interpretation, field geological and geomorphological surveys, drilling and trenching exposure, and magnetotelluric sounding, to systematically investigate the fault's spatial distribution, structural characteristics, and activity. The results indicate that the southern branch of the Jiali Fault Zone continuously extends along a line from south of Guxiang, through Gionala, Jinzhunongba, to Langqiunongba. Tectonic geomorphic evidence such as fault troughs, sag ponds, pressure ridges, bedrock fault mirrors, and horizontal slickensides were identified via remote sensing and field investigations. Magnetotelluric data revealed clear low-resistivity fracture zones, and drilling core samples exposed significant fault-related rocks. This evidence collectively confirms the existence and distribution of the fault within this segment. Combined with regional paleoseismic studies, it is concluded that this segment has the potential for Holocene activity. The research further systematically analyzed the deep engineering effects potentially triggered by fault activity, including surrounding rock deterioration, cosismic offset, high in-situ stress, seismic motion amplification, water inrush and mud gushing, geothermal anomalies, and secondary disasters at tunnel portals. The research results not only provide key geological constraints for improving the tectonic model of the southeastern Tibetan Plateau but also offer indispensable scientific basis for the planning, seismic design, and risk prevention and control of major projects (such as the Sichuan-Tibet Railway) traversing the fault zone.